{"created":"2023-06-19T07:18:47.309079+00:00","id":4377,"links":{},"metadata":{"_buckets":{"deposit":"208b447a-7843-4c20-8355-791f4f3b3573"},"_deposit":{"created_by":4,"id":"4377","owners":[4],"pid":{"revision_id":0,"type":"depid","value":"4377"},"status":"published"},"_oai":{"id":"oai:az.repo.nii.ac.jp:00004377","sets":["17:253:311","17:253:327","17:40:281","31:181:489"]},"author_link":["19107","19109","19116","19113","19112","19105","19108","19110","19114","19111","19106","19115"],"item_12_biblio_info_16":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2002-03-31","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"69","bibliographicPageStart":"59","bibliographicVolumeNumber":"3/4","bibliographic_titles":[{"bibliographic_title":"麻布大学雑誌"},{"bibliographic_title":"Journal of Azabu University","bibliographic_titleLang":"en"}]}]},"item_12_description_14":{"attribute_name":"Abstract","attribute_value_mlt":[{"subitem_description":"The genes, lmrA and lmrP, are identified as multidrug resistant genes in Lactococcus lactis subsp lactis MG1363, and these genes express LmrA and LmrP which are multidrug transporters (Konings et al., 1994-2000^<1-14)>). Multidrug transporters are a representative mechanism of multidrug resistance in bacteria and mammals. The amino acid sequence of LmrA showed 30% homology to the N- and C-terminal halves of the human multidrug resistant P-glycoprotein MDR1. And especially the sequence identities of ATP binding cassette domain of these protein are high (48% and 43% identical, respectively). In this study, attempts were made to confirm the presence of multidrug resistant genes in Lactococcus and Lactobacillus, which are lactic acid bacteria. The complete genomic DNA sequences of both lmrA and lmrP were isolated from 10 strains of Lactococcus lactis subsp. lactis by PCR amplification, whereas the presence of the homologous genomic DNA sequences genes was not confirmed in Lactococcus lactis subsp. cremoris and Lactobacillus. Vector pCAGGS (Niwa et al., 1991^<15)>) was used as the expression vector for lmrA in mammalian cells in this study. The PCR products of lmrA was cloned into pCAGGS in XhoI site, and pCAGGS-lmrA (7,873 bp) was constructed.","subitem_description_type":"Other"}]},"item_12_description_2":{"attribute_name":"ページ属性","attribute_value_mlt":[{"subitem_description":"P(論文)","subitem_description_type":"Other"}]},"item_12_description_3":{"attribute_name":"記事種別","attribute_value_mlt":[{"subitem_description":"原著論文","subitem_description_type":"Other"}]},"item_12_description_34":{"attribute_name":"format","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_12_description_4":{"attribute_name":"Type","attribute_value_mlt":[{"subitem_description":"ORIGINAL ARTICLE ","subitem_description_type":"Other"}]},"item_12_publisher_19":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"麻布大学"}]},"item_12_publisher_20":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_publisher":"Azabu University"}]},"item_12_source_id_21":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1346-5880","subitem_source_identifier_type":"ISSN"}]},"item_12_source_id_23":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA11561468","subitem_source_identifier_type":"NCID"}]},"item_12_text_10":{"attribute_name":"Author ID","attribute_value_mlt":[{"subitem_text_value":"000395"},{"subitem_text_value":"000247"},{"subitem_text_value":"-"},{"subitem_text_value":"-"},{"subitem_text_value":"000054"},{"subitem_text_value":"-"}]},"item_12_text_11":{"attribute_name":"所属機関","attribute_value_mlt":[{"subitem_text_value":"麻布大学獣医学部"},{"subitem_text_value":"麻布大学獣医学部"},{"subitem_text_value":"麻布大学獣医学部"},{"subitem_text_value":"宝酒造(株)遺伝子解析センター"},{"subitem_text_value":"麻布大学獣医学部"},{"subitem_text_value":"麻布大学獣医学部"}]},"item_12_text_12":{"attribute_name":"Institution or Company","attribute_value_mlt":[{"subitem_text_value":"School of Veterinary Medicine, Azabu University"},{"subitem_text_value":"School of Veterinary Medicine, Azabu University"},{"subitem_text_value":"School of Veterinary Medicine, Azabu University"},{"subitem_text_value":"Gene Analysis Center, Takara Syuzo Inc."},{"subitem_text_value":"School of Veterinary Medicine, Azabu University"},{"subitem_text_value":"School of Veterinary Medicine, Azabu University"}]},"item_12_text_9":{"attribute_name":"著者ID","attribute_value_mlt":[{"subitem_text_value":"000395"},{"subitem_text_value":"000247"},{"subitem_text_value":"-"},{"subitem_text_value":"-"},{"subitem_text_value":"000054"},{"subitem_text_value":"-"}]},"item_12_version_type_29":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_12_version_type_30":{"attribute_name":"text 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チカシ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"SUZUKI, Takehito","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"MORITA, Hidetoshi","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"MATSUSHITA, Rumi","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"HASHIZUME, Katsuhito","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"SAKATA, Ryoichi","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"TACHI, Chikashi","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2001-01-01"}],"displaytype":"detail","filename":"bull_jau_vol3-4-059.pdf","filesize":[{"value":"1.1 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"bull_jau_vol3-4-059","url":"https://az.repo.nii.ac.jp/record/4377/files/bull_jau_vol3-4-059.pdf"},"version_id":"bea6e881-444b-4a0d-a0a5-e1a94e90e252"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"multidrug resistance genes","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"LmrA and LmrP","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"lactic acid bacteria","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"expression vector","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin 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