{"created":"2023-06-19T07:18:32.209705+00:00","id":3923,"links":{},"metadata":{"_buckets":{"deposit":"114a6358-bb06-45cf-9d5d-ca65b40e3685"},"_deposit":{"created_by":4,"id":"3923","owners":[4],"pid":{"revision_id":0,"type":"depid","value":"3923"},"status":"published"},"_oai":{"id":"oai:az.repo.nii.ac.jp:00003923","sets":["17:254:334","31:181:468"]},"author_link":["18045","18044"],"item_12_biblio_info_16":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2009-03-31","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"214","bibliographicPageStart":"211","bibliographicVolumeNumber":"17/18","bibliographic_titles":[{"bibliographic_title":"麻布大学雑誌"},{"bibliographic_title":"Journal of Azabu University","bibliographic_titleLang":"en"}]}]},"item_12_description_13":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"犬血小板表面の免疫物質IgG，IgMおよび補体(C3)陽性コントロールの作製法を検討した。これらの血小板表面免疫物質は，FITCラベル抗犬IgG抗体，抗犬IgM抗体あるいは抗犬C3抗体を結合させ，フローサイトメトリーを用いて検出した。\n　IgG，IgMおよびC3それぞれ単独陽性血小板の作製を試みたが，得られたのはIgM陽性血小板のみであった。多血小板血漿にエンドトキシン(Lps)を加えたところ，IgG，IgMおよびC3いずれも陽性の血小板が得られた。このIgG/IgM/C3陽性血小板を，IgG，IgMおよびC3検出のいずれにも共通する陽性トロールとして使用することを考えた。そのためには，上記の3種FITCラベル抗体が，それぞれの標的物質に特異的に結合することが前提となる。\n　そこで，FITCラベル抗体の8倍量の非ラベル抗犬IgG，抗犬IgMあるいは抗犬C3抗体を10分前から作用させて結合をブロックしたところ，3種類のFITCラベル抗体は標的免疫物質のブロック処理によってのみ結合が阻害され，他の免疫物質との交差反応は否定された。\n　以上の結果から，これらの抗体を用いることを前提とすれば，IgG/IgM/C3陽性血小板は，3種の免疫物質のいずれにも共通する陽性コントロールとして利用できることが示唆された。","subitem_description_type":"Abstract"}]},"item_12_description_14":{"attribute_name":"Abstract","attribute_value_mlt":[{"subitem_description":"Different methods for the preparation of IgG-, IgM- and complement (C3)-positive canine platelets were investigated. Flow cytometry of FITC-conjugated anti-dog IgG, anti-dog IgM and anti-dog C3 polyclonal antibodies was used to detect platelet surface-bound immunological components.\n Platelets that were positive for IgM only were identified, but platelets positive for only either IgG or C3 were not found. Platelets positive for IgG, IgM and C3 (‘IgG/IgM/C3 positive platelets’) were prepared by incubating endotoxin (LPS) with platelet rich plasma (PRP) for 30 minutes at room temperature.\n We considered that IgG/IgM/C3 positive platelets could be used as a common positive control in IgG, IgM and C3 detection assays. To be a common positive control, FITC-conjugated antibodies must bind specifically to their targets on the IgG/IgM/C3 positive platelets. Preincubation of IgG/IgM/C3 positive platelets with eight times excess of non-labeled anti-dog IgG, IgM or C3 antibodies specifically blocked binding by FITC-conjugated anti-dog IgG, IgM or C3 antibodies.\n These results suggest that IgG/IgM/C3 positive platelets could be utilized as a common positive control in assays for the detection of platelet surface IgG, IgM and C3.","subitem_description_type":"Other"}]},"item_12_description_2":{"attribute_name":"ページ属性","attribute_value_mlt":[{"subitem_description":"P(論文)","subitem_description_type":"Other"}]},"item_12_description_24":{"attribute_name":"NII論文ID(NAID)","attribute_value_mlt":[{"subitem_description":"40016759592","subitem_description_type":"Other"}]},"item_12_description_3":{"attribute_name":"記事種別","attribute_value_mlt":[{"subitem_description":"特集","subitem_description_type":"Other"}]},"item_12_description_34":{"attribute_name":"format","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_12_description_4":{"attribute_name":"Type","attribute_value_mlt":[{"subitem_description":"FEATURE ARTICLES","subitem_description_type":"Other"}]},"item_12_publisher_19":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"麻布大学"}]},"item_12_publisher_20":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_publisher":"Azabu University"}]},"item_12_source_id_21":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1346-5880","subitem_source_identifier_type":"ISSN"}]},"item_12_source_id_23":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA11561468","subitem_source_identifier_type":"NCID"}]},"item_12_text_10":{"attribute_name":"Author ID","attribute_value_mlt":[{"subitem_text_value":"000071"}]},"item_12_text_11":{"attribute_name":"所属機関","attribute_value_mlt":[{"subitem_text_value":"麻布大学獣医学部"}]},"item_12_text_12":{"attribute_name":"Institution or Company","attribute_value_mlt":[{"subitem_text_value":"School or Veterinary Medicine, Azabu University"}]},"item_12_text_9":{"attribute_name":"著者ID","attribute_value_mlt":[{"subitem_text_value":"000071"}]},"item_12_version_type_29":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_12_version_type_30":{"attribute_name":"text version","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"土屋, 亮"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Tsuchiya, Ryo","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2014-04-17"}],"displaytype":"detail","filename":"bull_jau_vol17-18-211.pdf","filesize":[{"value":"324.2 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"bull_jau_vol17-18-211","url":"https://az.repo.nii.ac.jp/record/3923/files/bull_jau_vol17-18-211.pdf"},"version_id":"632d6b17-6643-4c01-a9b8-7cb51763d9a0"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"犬血球表面免疫物質検出法の改良","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"犬血球表面免疫物質検出法の改良"},{"subitem_title":"Modification for a detection method of canine blood cell surface IgG, IgM and complement","subitem_title_language":"en"}]},"item_type_id":"12","owner":"4","path":["334","468"],"pubdate":{"attribute_name":"公開日","attribute_value":"2014-02-21"},"publish_date":"2014-02-21","publish_status":"0","recid":"3923","relation_version_is_last":true,"title":["犬血球表面免疫物質検出法の改良"],"weko_creator_id":"4","weko_shared_id":4},"updated":"2023-12-19T05:58:12.148574+00:00"}