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アイテム
ネコカリシウイルスの分子疫学的研究
https://az.repo.nii.ac.jp/records/3921
https://az.repo.nii.ac.jp/records/3921bd742d57-6ee0-471a-bc10-d8f4955b7fc8
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
bull_jau_vol17-18-197 (373.2 kB)
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| Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| 公開日 | 2014-02-21 | |||||||||
| タイトル | ||||||||||
| タイトル | ネコカリシウイルスの分子疫学的研究 | |||||||||
| タイトル | ||||||||||
| タイトル | Study on the molecular epidemiology of Feline calicivirus | |||||||||
| 言語 | en | |||||||||
| 言語 | ||||||||||
| 言語 | jpn | |||||||||
| 資源タイプ | ||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||
| 資源タイプ | departmental bulletin paper | |||||||||
| ページ属性 | ||||||||||
| 内容記述タイプ | Other | |||||||||
| 内容記述 | P(論文) | |||||||||
| 記事種別 | ||||||||||
| 内容記述タイプ | Other | |||||||||
| 内容記述 | 特集 | |||||||||
| Type | ||||||||||
| 内容記述タイプ | Other | |||||||||
| 内容記述 | FEATURE ARTICLES | |||||||||
| 著者 |
原, 元宣
× 原, 元宣
× Hara, Motonobu
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| 著者ID | ||||||||||
| 000039 | ||||||||||
| Author ID | ||||||||||
| 000039 | ||||||||||
| 所属機関 | ||||||||||
| 麻布大学獣医学部獣医学科微生物学第二研究室 | ||||||||||
| Institution or Company | ||||||||||
| Department of Microbiology II, School of Veterinary Medicine, Azabu University | ||||||||||
| 抄録 | ||||||||||
| 内容記述タイプ | Abstract | |||||||||
| 内容記述 | FCV感染症の分子疫学的検討を行うため，カプシド領域におけるFCV検出用プライマーの検討を試みた。新たに作成した3組のプライマーについて，CP-S/CP-Aで検出率が若干低い値を示したが，8F-S/8F-AとAo-S/Ao-Aにおいては既報のプライマーよりも増幅領域が広く，またGI GIIどちらの群に属する株も100%検出できたので，この2種類のプライマーを用い遺伝子群の判定を行えると期待される。 | |||||||||
| Abstract | ||||||||||
| 内容記述タイプ | Other | |||||||||
| 内容記述 | FCV comprises only a single serotype, but many mutations are present in the gene sequence, forming quasispecies consisting of various molecular species of sequences. Improvement of the target detection accuracy by RT-PCR is very important for molecular biological approaches. FCV has been reported to be divided into 2 groups, GI and GII, based on the capsid region (bases 6419-7053). We performed RT-PCR using Seal-S/Seal-A primers, as used for grouping in previous reports, and identified the presence of undetectable field strains. To widely detect field isolates using the capsid region, we prepared new primers consisting of sequences outside the reported primer sequences, and investigated the detection accuracy. Eight (including 3 strains isolated from vaccinated cats) of 30 FCV strains (27.0 %) were not detectable using Seal-S/Seal-A primers. The detection rates with the new primers were: 8F-S/8R-A, 100 %; AO-S/AO-A, 100 %; and CP-S/CP-A, 96.7 %. Undetectable strains had marked mutations in the primer binding sites. The mutation rate of the Seal-S binding site was 29.2 %, whereas the rate was 6.9 % in detectable strains. Seven of the 8 undetectable strains belonged to GII, which consists of only Japanese strains, showing a regional influence. Since the undetectable stains included 3 isolates (37.5 %) from vaccinated cats, the presence of such vaccine breakdown strains with marked antigen mutation in vaccinated cats should be considered in clinical diagnosis by PCR. Of the 3 pairs of primers prepared, 8F-S/8R-A and AO-S/AO-A primers amplified wider regions than the reported primers, and detected 100 % of GI and GII strains, suggesting the usefulness of these as detection primers for FCV molecular epidemiology. | |||||||||
| 書誌情報 |
麻布大学雑誌 en : Journal of Azabu University 巻 17/18, p. 197-201, 発行日 2009-03-31 |
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| 出版者 | ||||||||||
| 出版者 | 麻布大学 | |||||||||
| Publisher | ||||||||||
| 出版者 | Azabu University | |||||||||
| ISSN | ||||||||||
| 収録物識別子タイプ | ISSN | |||||||||
| 収録物識別子 | 1346-5880 | |||||||||
| 書誌レコードID | ||||||||||
| 収録物識別子タイプ | NCID | |||||||||
| 収録物識別子 | AA11561468 | |||||||||
| NII論文ID(NAID) | ||||||||||
| 内容記述タイプ | Other | |||||||||
| 内容記述 | 40016759590 | |||||||||
| 著者版フラグ | ||||||||||
| 出版タイプ | VoR | |||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||
| text version | ||||||||||
| 出版タイプ | VoR | |||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||
| format | ||||||||||
| 内容記述タイプ | Other | |||||||||
| 内容記述 | application/pdf | |||||||||
