{"created":"2023-06-19T07:18:31.975930+00:00","id":3918,"links":{},"metadata":{"_buckets":{"deposit":"67b3c04e-5c89-400e-9f82-ab5fe18f238f"},"_deposit":{"created_by":4,"id":"3918","owners":[4],"pid":{"revision_id":0,"type":"depid","value":"3918"},"status":"published"},"_oai":{"id":"oai:az.repo.nii.ac.jp:00003918","sets":["17:40:278","31:181:468"]},"author_link":["18015","18014"],"item_12_biblio_info_16":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2009-03-31","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"190","bibliographicPageStart":"189","bibliographicVolumeNumber":"17/18","bibliographic_titles":[{"bibliographic_title":"麻布大学雑誌"},{"bibliographic_title":"Journal of Azabu University","bibliographic_titleLang":"en"}]}]},"item_12_description_13":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"遺伝子の働きを多様にする重要なステップであるRNAスプライシングの様子を蛍光・発光タンパクを利用して可視化・定量化する技術を開発し改良を加えた。微小管結合タンパク質タウのエキソン(2, 3, 10)を持つスプライシングベクターを作製し，各エキソンの選択的スプライシングについて詳細な解析を進めている。","subitem_description_type":"Abstract"}]},"item_12_description_14":{"attribute_name":"Abstract","attribute_value_mlt":[{"subitem_description":"Alternative splicing that contributes to proteomic diversity is a mechanism by which multiple transcripts are generated from a transcript from single gene. This post-transcriptional processing may occur in over 70 % of human genes. Abnormal splicing is associated with some disease, such as Alzheimer's disease (AD) and FTDP-17. Microtubule associated protein tau (MAPT) is produced as six alternative splicing isoforms in brain. Abnormality in this splicing has been thought as a causative event through which AD and FTDP-17 might be developed and/or progressed. Hence a change in alternative splicing must be worth monitoring for diagnostics and therapy of the diseases. In this study, we tried to develop a method for visualizing alternative splicing of tau pre-mRNA. Using the splicing cassette vector. pGL3intl and pGL3intl-MCS. we have developed, we constructed plasmids containing tau exon (exon 2, 3 or 10) and observed the alternative splicing of each exon in cultured cells such as COS7. SH-SY5Y or HEK293.","subitem_description_type":"Other"}]},"item_12_description_2":{"attribute_name":"ページ属性","attribute_value_mlt":[{"subitem_description":"P(論文)","subitem_description_type":"Other"}]},"item_12_description_3":{"attribute_name":"記事種別","attribute_value_mlt":[{"subitem_description":"特集","subitem_description_type":"Other"}]},"item_12_description_34":{"attribute_name":"format","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_12_description_4":{"attribute_name":"Type","attribute_value_mlt":[{"subitem_description":"FEATURE ARTICLES","subitem_description_type":"Other"}]},"item_12_publisher_19":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"麻布大学"}]},"item_12_publisher_20":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_publisher":"Azabu University"}]},"item_12_source_id_21":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1346-5880","subitem_source_identifier_type":"ISSN"}]},"item_12_source_id_23":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA11561468","subitem_source_identifier_type":"NCID"}]},"item_12_text_10":{"attribute_name":"Author ID","attribute_value_mlt":[{"subitem_text_value":"000298"}]},"item_12_text_11":{"attribute_name":"所属機関","attribute_value_mlt":[{"subitem_text_value":"麻布大学生命・環境科学部食品生命科学科遺伝子科学研究室"}]},"item_12_text_12":{"attribute_name":"Institution or Company","attribute_value_mlt":[{"subitem_text_value":"Lab. of Molecular Biology, Dept. of Food and Lire Science School of Life and Environmental Science, Azabu University"}]},"item_12_text_9":{"attribute_name":"著者ID","attribute_value_mlt":[{"subitem_text_value":"000298"}]},"item_12_version_type_29":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_12_version_type_30":{"attribute_name":"text version","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"村山, 洋"}],"nameIdentifiers":[{"nameIdentifier":"18014","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Murayama, Ohoshi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"18015","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2014-04-17"}],"displaytype":"detail","filename":"bull_jau_vol17-18-189.pdf","filesize":[{"value":"850.4 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"bull_jau_vol17-18-189","url":"https://az.repo.nii.ac.jp/record/3918/files/bull_jau_vol17-18-189.pdf"},"version_id":"813bf179-8727-484c-9935-10227c6565eb"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"新規RNAスプライシング検出システム","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"新規RNAスプライシング検出システム"},{"subitem_title":"Methods for quantification of RNA splicing","subitem_title_language":"en"}]},"item_type_id":"12","owner":"4","path":["278","468"],"pubdate":{"attribute_name":"公開日","attribute_value":"2014-02-21"},"publish_date":"2014-02-21","publish_status":"0","recid":"3918","relation_version_is_last":true,"title":["新規RNAスプライシング検出システム"],"weko_creator_id":"4","weko_shared_id":4},"updated":"2023-06-19T08:23:10.557262+00:00"}