@article{oai:az.repo.nii.ac.jp:00003917,
 author = {斑目, 広郎 and 西野, 佳以 and 井上, 真紀 and Madarame, Hiroo and Nishino, Yoshii and Inoue, Maki},
 journal = {麻布大学雑誌, Journal of Azabu University},
 month = {Mar},
 note = {1. 実験1および2においてCRP3株感染ラットは発症せず，CRNP5株は全例発症し，観察期間中に全例が死亡または重篤な症状(スコア3)となった。
2. CRP3株およびCRNP5株感染ラットの脳では全例で，海馬病変を主体とする非炎症性の脳病変(脳症)が認められた。
3. CRNP5株感染ラットでは大脳皮質菲薄化を伴う脳室拡張(hydrocephalus ex vacuo)が認められた。
4. CRP3株とCRNP5株感染ラットの脳では神経細胞を主体とするBDV陽性所見が存在していた。
5. 偽接種群のヌードラットは実験1及び2において臨床症状が認められず組織学的に正常であった。
6. 脳を大脳皮質，脳幹部及び小脳に分割してウイルス力価を測定したところ，BDV-CRP3株感染群に比べBDV-CRNP5株感染群では脳幹部のウイルス力価が高かった。
7. ボルナ病ウイルス感染ではT細胞性免疫応答を介さない発症機序があり，発症程度及び病変はウイルス株により異なることが示唆された。, Fifty-three immunodeficient nude rats (four weeks old) were used in this experiment. The rats were inoculated with either CRNP5 strain or CRP3 strain of Borna disease virus (BDV) intracranially and were observed for seven weeks (experiment 1) or eight weeks (experiment 2). In experiment 1, if the inoculated rats showed clinical symptoms and developed severe Borna disease (BD) (score 3). the rats were killed and histological studies and virus titration of the brain samples were performed. If the rats did not show severe clinical symptoms, the rats were killed and histological studies and virus titration of the brain samples were performed at the end of the experiment. In experiment 2, the inoculated rats were killed chronologically in the second, fourth or eighth week after inoculation or when the inoculated rats showed clinical symptoms and developed severe BD (score 3).
All CRNP5 inoculated rats developed neurological signs, suspected of indicating BD between 24 and 36 days after virus inoculation. Conversely, CRP3 inoculated rats did not develop any neurological signs during the course of either experiment.
Histopathologically and immunohistochemically there were three common findings in both strains of BDV inoculated rats: 1) degeneration. necrosis and apoptosis of nerve cells and focal calcifications without inflammation, 2) gliosis, 3) BDV antigen positive reactions in the nerve cells and astrocytes. In CRP3 inoculated rats, atrophy of the dentate gyrus associated with degeneration, necrosis and apoptosis of the neurons of the granular layers of the dentate gyrus was the significant finding. In CRNP5 inoculated rats. there were three major changes: 1 ) hydrocephalus ex vacuo, 2) disarrangements of the nerve cells in the hippocampal CA3 region and degeneration. necrosis and apoptosis of the nerve cells of the granular layers of the dentate gyrus. In the brain samples of all inoculated nude rats. the virus was recovered and the titer of the virus was higher than other parts of the brain.
In the previous athymic rats infections of BDV. the virus-specific nucleic acid and infectious virus were found in the inoculated rats. but there were no signs of BD and histopathological lesions., P(論文), 40016759587, 特集, application/pdf, FEATURE ARTICLES},
 pages = {185--188},
 title = {ヌードラットにおけるボルナ病ウイルス感染},
 volume = {17/18},
 year = {2009}
}