{"created":"2023-06-19T07:18:07.291303+00:00","id":3244,"links":{},"metadata":{"_buckets":{"deposit":"6c787abc-fa42-44ce-a688-4d0f6a61e6d0"},"_deposit":{"created_by":4,"id":"3244","owners":[4],"pid":{"revision_id":0,"type":"depid","value":"3244"},"status":"published"},"_oai":{"id":"oai:az.repo.nii.ac.jp:00003244","sets":["370:15:392"]},"author_link":["16318","16319"],"item_10006_date_granted_11":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2003-05-21"}]},"item_10006_degree_grantor_9":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_name":"麻布大学"}]}]},"item_10006_degree_name_8":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士(獣医学)"}]},"item_10006_description_22":{"attribute_name":"Abstract","attribute_value_mlt":[{"subitem_description":"Feline calicivirus(FCV) causes an acute, highly contagious disease in cats especially in kitten. That is called the FCV infection characterized by upper respiratory disease, pneumonia, ulcerative stomatitis or gingivitis, and occasionally, enteritis or arthritis. The FCV particle is recognized by nonenveloped icosahedral symmetry with a diameter of 27 to 40 nm. The virion consists of a positive single-strand of nonsegmented RNA with 32 cup-shaped depressions on the capsid. The capsid is composed of a single major polypeptide, and serologically, the FCV is regarded as belonging to one single serotype.\n The molecular epidemiological study of FCV was performed and induced a genotypic classfication for Japanese and foreign strains by phylogenetic analyses of the nucleotide sequences and amino acid sequence(370~580) of the capsid-coding regions(B through F) with examination of their isolations, ages, locality, pathogenicity and serological properties. These results suggest usefulness of developing a norvel vaccines directed against strains for which conventional vaccine has limited effectiveness.\n\n1. Genotyping\n1-1 Genotype grouping\n The construction of a phylogenetic tree for 77 strains divided FCVs into two distinct groups of genes. Of the 77 strains, 69% and 31% belonged to gene group I and II, respectively. Their bootstrap values were high at 96.5%, showing that both gene groups were divided into two genotypes with a high probability.\n Gene group I formed clusters consisting of many branches, but their low bootstrap values did not allow any of them to be distinguished from gene group I, whereas gene group II indicated a bootstrap value of 99.7% and formed two gene subgroups(IIa 17%, IIb 14%).\n1-2 Phylogenetic analysis of amino acid sequences\n A phylogenetic tree was constructed by converting nucleotide sequences into amino acid sequences, which were broadly divided into two gene groups(IA, IIA) consisting of similar members as in the phylogenetic tree of nucleotide sequences. Since the IIa and IIb subgroups could not be clearly distinguished bacause of a low bootstrap value of 53.6%, they were regarded as the same gene group. These results indicate that nucleotide sequence-based analysis is more sensitive in analyzing genotypes.\n1-3 Comparison of amino acids\n Multivariate analysis of the amino acids common to group I or II showed that gene groups I and II differed in two amino acid residues, that is, the amino acids at position 539 in the F region were alanine or proline in group I and valine or phenylalanine in group II, and those at position 557 were glutamine and serine, respectively.\n2. Epidemiological analysis\n2-1 Comparison of distribution by country\n Genotype classification of FCVs by country of origin showed that the foreign strains(German, English, Canadian, USA and Australian) and about half of the Japanese strains belonged to gene group I, while gene group II consisted only of Japanese strains without any foreign strains. There was thus a clear difference between the two groups(p