| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2026-03-10 |
| タイトル |
|
|
タイトル |
Development of a novel fluorogenic assay method for screening inhibitors of bovine leukemia virus (BLV) protease and identification of mitorubrinic acid as an anti-BLV compound |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 著者 |
Murakami, Hironobu
Fujikawa, Yuuta
Mori, Masaya
Mosu, Nozomi
Taguchi, Akihiro
Hayashi, Yoshio
Inoue, Hideshi
Kamisuki, Shinji
|
| Abstract |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Bovine leukemia virus (BLV) causes enzootic bovine leukosis, a fatal cattle disease that leads to significant economic losses in the livestock industry. Currently, no effective BLV countermeasures exist, except testing and culling. In this study, we developed a high-throughput fluorogenic assay to evaluate the inhibitory activity of various compounds on BLV protease, an essential enzyme for viral replication. The developed assay method was used to screen a chemical library, and mitorubrinic acid was identified as a BLV protease inhibitor that exhibited stronger inhibitory activity than amprenavir. Additionally, the anti-BLV activity of both the compounds was evaluated using a cell-based assay, and mitorubrinic acid was found to exhibit inhibitory activity without cytotoxicity. This study presents the first report of a natural inhibitor of BLV protease-mitorubrinic acid-a potential candidate for the development of anti-BLV drugs. The developed method can be used for high-throughput screening of large-scale chemical libraries. |
|
言語 |
en |
| 書誌情報 |
Bioscience, Biotechnology, and Biochemistry
巻 87,
号 9,
p. 946-953,
発行日 2023-06-06
|
| 出版者 |
|
|
出版者 |
Oxford University Press (OUP) |
| DOI |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
10.1093/bbb/zbad073 |
| 著者版フラグ |
|
|
出版タイプ |
AM |
|
出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
| 査読 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
査読あり |
BLV_probe_BBB.pdf (587 KB)
