| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2026-02-25 |
| タイトル |
|
|
タイトル |
Isolation and whole-genome sequencing of a novel aviadenovirus from owls in Japan |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 著者 |
Kobayashi, Hiroko
Uchida, Yu
Fujino, Kan
Horie, Masayuki
Umezawa, Eisuke
Aihara, Naoyuki
Kamiie, Junichi
Shimoda, Hiroshi
Maeda, Ken
Une, Yumi
Taharaguchi, Satoshi
|
| Abstract |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Adenoviruses have been reported to infect a variety of birds. Here, we isolated a novel adenovirus from the liver of a dead owl chick (Bengal eagle owl; Bubo bengalensis) at a raptor-breeding facility in Japan and determined the complete genome sequence of the virus. We performed necropsies on the dead owl chicks and found that they had enlarged livers, pericardial edema, and focal necrosis of the liver tissue. Transmission electron microscopy of the liver tissue revealed a virus-like structure, appearing as paracrystalline arrays in the nucleus, and immunohistochemical staining with anti-adenovirus antibodies showed positive reactions in hepatocytes and other cells. Attempts to isolate the virus from homogenized liver tissue of a dead owl chick showed a cytopathic effect on chicken-derived cultured cells after multiple blind passages. Further, we determined the complete genome sequence of this virus and performed phylogenetic analysis, revealing that this adenovirus belongs to the genus Aviadenovirus, forming a cluster with fowl and turkey aviadenoviruses. The amino acid sequence divergence between the DNA polymerase of this virus and its closest known adenovirus relative is approximately 29%, implying that this virus can be assigned to a new species in the genus Aviadenovirus. Based on our data, this novel owl adenovirus is a likely cause of fatal infections in owls, which may threaten wild and captive owl populations. Further, this virus is unique among raptor adenoviruses in that it infects chicken-derived cultured cells, raising the importance of further investigations to evaluate interspecies transmission of this virus. |
|
言語 |
en |
| 書誌情報 |
Archives of Virology
巻 167,
号 3,
p. 829-838,
発行日 2022-02-03
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| 出版者 |
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|
出版者 |
Springer Nature |
| DOI |
|
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|
識別子タイプ |
DOI |
|
|
関連識別子 |
10.1007/s00705-022-05380-3 |
| 著者版フラグ |
|
|
出版タイプ |
AM |
|
出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
| 査読 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
査読あり |
260213_OlAdV_revised_manuscript.pdf (720 KB)
