| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2026-02-25 |
| タイトル |
|
|
タイトル |
Isolation of the canine inhibin βB subunit gene and characterization of signalling mediated by canine inhibin βB |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 著者 |
Sakota, Shotaro
Shimokawa, Fumie
Funaba, Masayuki
Murakami, Masaru
|
| Abstract |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Activin B, a homodimer of the inhibin βB subunit, acts as a regulator of gonadal function and as an adipokine. To clarify the role of activin B in dogs, we characterized the canine inhibin βB gene and signalling pathways regulated by the canine inhibin βB. Using 5'- and 3'-rapid amplification of cDNA end (RACE) and RT-PCR on RNA isolated from the ovary of dogs, we identified short and long forms of the inhibin βB gene. Immunoreactive inhibin βB molecules were detected at ~25 and ~14 kDa under nonreducing and reducing conditions, respectively, in culture supernatants from HEK293 cells transfected with a plasmid containing the long form of the inhibin βB gene, indicating activin B production and secretion. Similar to human and murine activin B, the canine activin B-stimulated transcriptions of reporter genes, CAGA-luc and Hepcidin-luc, regulated by the canonical activin/transforming growth factor-β (TGF-β) and bone morphogenetic protein (BMP) pathway, respectively. Activin B-induced CAGA-luc transcription was not detected in ALK7-deficient MDCK canine-derived cells; however, the forced expression of ALK7 resulted in the activin B-dependent expression in MDCK cells. Unexpectedly, the activin B-induced activation of the BMP pathway was partially blocked by the inhibition of endogenous activin/TGF-β receptor activity. The present study identified an experimentally isolated long form of the canine inhibin βB gene producing activin B that transactivates BMP- and activin/TGF-β-regulated gene expression. |
|
言語 |
en |
| 書誌情報 |
Cell Biochemistry and Function
巻 39,
号 8,
p. 970-982,
発行日 2021-08-12
|
| 出版者 |
|
|
出版者 |
Wiley |
| DOI |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
10.1002/cbf.3664 |
| 著者版フラグ |
|
|
出版タイプ |
AM |
|
出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
| 査読 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
査読あり |
Manuscript (Sakota)_cor.pdf (1.6 MB)
