| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2025-03-14 |
| タイトル |
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|
タイトル |
Guest edited collection serological study of SARS-CoV-2 antibodies in japanese cats using protein-A/G-based ELISA |
|
言語 |
en |
| 言語 |
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言語 |
eng |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 著者 |
Imanishi, Ichiro
Asahina, Ryota
Hayashi, Shunji
Uchiyama, Jumpei
Hisasue, Masaharu
Yamasaki, Masahiro
Murata, Yoshiteru
Morikawa, Shigeru
Mizutani, Tetsuya
Sakaguchi, Masahiro
|
| Abstract |
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内容記述タイプ |
Abstract |
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内容記述 |
Background
Little is known about the epidemic status of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in cats in Japan due to insufficiently reliable seroepidemiological analysis methods that are easy to use in cats.
Results
We developed a protein-A/G-based enzyme-linked immunosorbent assay (ELISA) to detect antibodies against SARS-CoV-2 in cats. The assay was standardized using positive rabbit antibodies against SARS-CoV-2. The ELISA results were consistent with those of a conventional anti-feline-immunoglobulin-G (IgG)-based ELISA. To test the protein-A/G-based ELISA, we collected blood samples from 1,969 cats that had been taken to veterinary clinics in Japan from June to July 2020 and determined the presence of anti-SARS-CoV-2 antibodies. Nine cats were found to have SARS-CoV-2 S1-specific IgG, of which 4 had recombinant receptor-binding domain-specific IgG. Of those 9 samples, one showed neutralizing activity. Based on these findings, we estimated that the prevalence of SARS-CoV-2 neutralizing antibodies in cats in Japan was 0.05% (1/1,969 samples). This prevalence was consistent with the prevalence of neutralizing antibodies against SARS-CoV-2 in humans in Japan according to research conducted at that time.
Conclusions
Protein-A/G-based ELISA has the potential to be a standardized method for measuring anti-SARS-CoV-2 antibodies in cats. The infection status of SARS-CoV-2 in cats in Japan might be linked to that in humans. |
|
言語 |
en |
| bibliographic_information |
BMC veterinary research
巻 18,
号 1,
p. 443-443,
発行日 2022-12-21
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| 出版者 |
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出版者 |
Springer Nature |
| item_10002_relation_14 |
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識別子タイプ |
DOI |
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関連識別子 |
10.1186/s12917-022-03527-7 |
| 出版タイプ |
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|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| item_1730428627434 |
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|
内容記述タイプ |
Other |
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内容記述 |
査読あり |
Guest edited collection serological study of SARS-CoV-2 antibodies in japanese cats using protein-A_G-based ELISA.pdf (1.2 MB)
