| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2025-03-14 |
| タイトル |
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|
タイトル |
Extracellular Vesicles Derived From Canine Mesenchymal Stromal Cells in Serum Free Culture Medium Have Anti-inflammatory Effect on Microglial Cells |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 著者 |
Kuwahara, Yukina
Yoshizaki, Karin
Nishida, Hidetaka
Kamishina, Hiroaki
Maeda, Sadatoshi
Takano, Katsura
Fujita, Naoki
Nishimura, Ryohei
Jo, Jun-Ichiro
Tabata, Yasuhiko
Akiyoshi, Hideo
|
| Abstract |
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内容記述タイプ |
Abstract |
|
内容記述 |
Mesenchymal stem/stromal cells (MSCs) have been used as cell sources for treating dogs with naturally-occurring diseases. Extracellular vesicles (EVs) derived from MSCs are now recognized as pivotal to modulating the immune response and supporting tissue repair. Manufacture of MSC-EVs for clinical application mandates removal of the xeno-proteins, including fetal bovine serum. The objective of this study was to examine whether canine MSCs survived and secreted EVs in serum-free medium (SFM) conditions and to assess the immunomodulatory effect of EVs in vitro. Canine MSCs were found to survive and secrete EVs under SFM conditions. The surface markers of MSCs in the SFM were similar to MSCs in complete culture medium. Canine MSC-EVs had a diameter of ~300 nm and were positive for EV markers. MSC-derived EVs from the serum-free condition reduced the levels of IL-1β by BV-2 cells in response to LPS stimulation. These results warrant further studies of the use of SFM for producing EVs derived from canine MSCs. |
|
言語 |
en |
| bibliographic_information |
Frontiers in veterinary science
巻 8,
p. 633426-633426,
発行日 2021-04-28
|
| 出版者 |
|
|
出版者 |
Frontiers Media SA |
| item_10002_relation_14 |
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|
識別子タイプ |
DOI |
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|
関連識別子 |
10.3389/fvets.2021.633426 |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| item_1730428627434 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
査読あり |
fvets-08-633426.pdf (1.4 MB)
