| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2025-03-14 |
| タイトル |
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|
タイトル |
Human placental hydrolysate promotes the long-term culture of hepatocyte-like cells derived from canine bone marrow |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 著者 |
Neo, Sakurako
Makiishi, Eri
Fujimoto, Ayumi
Hisasue, Masaharu
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| Abstract |
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内容記述タイプ |
Abstract |
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内容記述 |
Long-term culture of canine artificial hepatocytes has not been established. We hypothesized that human placental hydrolysate (hPH) may support the long-term culture of differentiated hepatocyte-like cells. Canine bone marrow cells were cultured using modified hepatocyte growth medium supplemented with hPH. Quantitative reverse transcription polymerase chain reaction (RT-PCR) and immunocytochemical analysis for albumin, qualitative RT-PCR for cytochrome P450 1A1 (CYP1A1), hepatocyte growth factor (HGF), Cytokeratin 7 (CK7), CD90, CD44, and CD34, and functional analyses of CYP450 activity and low-density lipoprotein (LDL) uptake were performed. Cultured hepatocyte-like cells were able to maintain hepatocyte characteristics, including morphology, albumin synthesis, CYP450 activity, and LDL uptake for 80 days. Thus, hPH may be a potential facilitator for the long-term culture of hepatocyte-like cells. Clinicopathologically, this culture protocol of artificial hepatocytes will contribute to liver function evaluation. |
|
言語 |
en |
| bibliographic_information |
The Journal of Veterinary Medical Science
巻 82,
号 12,
p. 1821-1827,
発行日 2021-01-05
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| 出版者 |
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|
出版者 |
The Japanese Society of Veterinary Science |
| item_10002_source_id_9 |
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収録物識別子タイプ |
ISSN |
|
収録物識別子 |
0916-7250 |
| item_10002_relation_14 |
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|
識別子タイプ |
DOI |
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関連識別子 |
10.1292/jvms.20-0320 |
| 出版タイプ |
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|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| item_1730428627434 |
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|
内容記述タイプ |
Other |
|
内容記述 |
査読あり |
82_20-0320.pdf (1.9 MB)
