| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2025-03-14 |
| タイトル |
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タイトル |
Functional analysis of CD44 variants and xCT in canine tumours |
|
言語 |
en |
| 言語 |
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|
言語 |
eng |
| 資源タイプ |
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|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 著者 |
Tanabe, Atsushi
Kimura, Kento
Tazawa, Hana
Maruo, Takuya
Taguchi, Masayuki
Sahara, Hiroeki
|
| Abstract |
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内容記述タイプ |
Abstract |
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内容記述 |
The cell surface glycoprotein CD44 has various types of splicing variants, which contribute to its multiple distinct cellular functions. Recently, it was reported that the CD44v8-10 isoform interacts with the system Xc(-) transporter-related protein (xCT), and inhibits the accumulation of reactive oxygen species by promoting the synthesis of the antioxidant glutathione in human tumour cells. In this study, we investigated the expression and function of CD44 variants and xCT in canine tumours. From semi-quantitative reverse transcription polymerase chain reaction analysis, the mRNA expression of the CD44v8-10 isoform was observed in canine tumour tissues as well as human cases. The overexpression of CD44v8-10 may promote the synthesis of glutathione and enhance the resistance to radiation of canine breast tumour cells. Furthermore, canine xCT mRNA expression was significantly upregulated in the canine breast tumour tissues as compared to the normal tissues surrounding the tumours. To investigate the function of canine xCT, we treated canine tumour cells with the xCT inhibitor sulfasalazine. Consequently, the sulfasalazine-treated cells were more sensitive to oxidative stress than the non-treated cells. Taken together, these results suggested that CD44v8-10 and xCT play important roles in the therapy resistance of canine tumours as well as human tumours. |
|
言語 |
en |
| bibliographic_information |
Veterinary Medicine and Science
巻 7,
号 2,
p. 577-585,
発行日 2021-03
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| 出版者 |
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出版者 |
Wiley |
| item_10002_source_id_9 |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
2053-1095 |
| item_10002_relation_14 |
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識別子タイプ |
DOI |
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関連識別子 |
10.1002/vms3.397 |
| 出版タイプ |
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出版タイプ |
VoR |
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出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| item_1730428627434 |
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内容記述タイプ |
Other |
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内容記述 |
査読あり |
Veterinary Medicine Sci - 2020 - Tanabe - Functional analysis of CD44 variants and xCT in canine tumours.pdf (755 KB)
