| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2025-03-14 |
| タイトル |
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タイトル |
Highly successful production of viable mice derived from vitrified germinal vesicle oocytes |
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言語 |
en |
| 言語 |
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|
言語 |
eng |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 著者 |
Kamoshita, Maki
Fujiwara, Katsuyoshi
Ito, Junya
Kashiwazaki, Naomi
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| Abstract |
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内容記述タイプ |
Abstract |
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内容記述 |
The vitrification of immature germinal vesicle (GV) oocytes is an important way to preserve genetic resources and female fertility. However, it is well known that cryopreserved GV oocytes have very poor developmental ability and that further improvement in this technique is needed. We previously reported the successful vitrification of matured mouse oocytes with enclosed cumulus cells using the calcium-free vitrification solution supplemented with ethylene glycol (EG) by the minimal volume cooling (MVC) method. In this study, we investigated whether our method is applicable to the vitrification of mouse oocytes at the GV stage (GV oocytes). Following maturation and fertilization in vitro, vitrified GV oocytes showed high survival (94.3 ± 2.0%) and maturation (94.3 ± 2.1%) rates. Although the fertilization and blastocyst rates of vitrified oocytes (fertilization: 46.6 ± 4.9% and blastocyst: 46.6 ± 3.0%) were significantly lower than those of fresh oocytes (fertilization: 73.0 ± 7.1% and blastocyst: 71.6 ± 8.0%) (P < 0.01), there were no differences in the ability to develop to term between fresh oocytes (50.0 ± 8.4%) and vitrified oocytes (37.5 ± 4.6%) (P > 0.05). In conclusion, we here show, for the first time, the efficient production of live mice derived from vitrified GV oocytes. |
|
言語 |
en |
| bibliographic_information |
PLOS ONE
巻 16,
号 3,
p. e0248050-e0248050,
発行日 2021-03-11
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| 出版者 |
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出版者 |
Public Library of Science (PLoS) |
| item_10002_relation_14 |
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識別子タイプ |
DOI |
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関連識別子 |
10.1371/journal.pone.0248050 |
| 出版タイプ |
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出版タイプ |
VoR |
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出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| item_1730428627434 |
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内容記述タイプ |
Other |
|
内容記述 |
査読あり |
journal.pone.0248050.pdf (721 KB)
