| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2025-03-14 |
| タイトル |
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タイトル |
Validation of a newly generated oxytocin antibody for enzyme-linked immunosorbent assays |
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言語 |
en |
| 言語 |
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|
言語 |
eng |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 著者 |
Murata, Kaori
Nagasawa, Miho
Onaka, Tatsushi
Takeyama, Ken-Ichi
Kikusui, Takefumi
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| Abstract |
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内容記述タイプ |
Abstract |
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内容記述 |
The biological and psychological significance of oxytocin is increasingly recognized; however, reliable assays of oxytocin in biological samples have not been developed. We raised a new oxytocin polyclonal rabbit antibody against synthetic oxytocin. The affinity of antibodies to oxytocin was examined by a radio-immunoassay and compared with that of a previously validated antibody. One antibody showed affinity for oxytocin in the radio-immunoassay. We developed a solid-phase ELISA for oxytocin using this antibody and compared it with existing methods. The newly developed ELISA showed comparable results using urine samples but not using serum samples. These results indicate that the new ELISA is useful for urinary oxytocin; further modifications, such as different extraction methods, are needed for its application to serum oxytocin. |
|
言語 |
en |
| bibliographic_information |
The Journal of Veterinary Medical Science
巻 83,
号 3,
p. 478-481,
発行日 2021-04-03
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| 出版者 |
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出版者 |
The Japanese Society of Veterinary Science |
| item_10002_relation_14 |
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識別子タイプ |
DOI |
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関連識別子 |
10.1292/jvms.20-0723 |
| 出版タイプ |
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出版タイプ |
VoR |
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出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| item_1730428627434 |
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内容記述タイプ |
Other |
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内容記述 |
査読あり |
83_20-0723.pdf (1022 KB)
