| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2025-02-28 |
| タイトル |
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タイトル |
Promoter CpG methylation inhibits Krüppel-like factor 2 (KLF2)-Mediated repression of hTERT gene expression in human T-cells |
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言語 |
en |
| 言語 |
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|
言語 |
eng |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 著者 |
Mizuguchi, Mariko
Hara, Toshifumi
Yoshita-Takahashi, Manami
Kohda, Takashi
Tanaka, Yuetsu
Nakamura, Masataka
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| Abstract |
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内容記述タイプ |
Abstract |
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内容記述 |
Constitutive expression of human telomerase reverse transcriptase (hTERT) with DNA methylation of its promoter is a common phenomenon in tumor cells. We recently found that the transcriptional factor Krüppel-like factor 2 (KLF2) binds to the CpG sequences in the hTERT promoter and inhibits hTERT gene expression in normal resting T-cells. The human T-cell line Kit 225 in the resting phase induced by the deprivation of interleukin (IL)-2 showed no decrease in the expression of hTERT, despite the high expression of KLF2. To elucidate the mechanisms of deregulation of hTERT expression in T-cells, we examined the relationship between DNA methylation and KLF2 binding to the hTERT promoter. The hTERT promoter was methylated in Kit 225 cells, resulting in the inhibition of the binding of KLF2 to the promoter. DNA demethylation by the reagent Zebularine recovered KLF2 binding to the hTERT promoter, followed by the downregulation of its gene expression. These findings indicate that the repressive effect of KLF2 on hTERT gene expression is abolished by DNA methylation in T-cell lines. |
|
言語 |
en |
| bibliographic_information |
Biochemistry and Biophysics Reports
巻 26,
p. 100984-100984,
発行日 2021-07
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| 出版者 |
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出版者 |
Elsevier BV |
| item_10002_source_id_9 |
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収録物識別子タイプ |
ISSN |
|
収録物識別子 |
2405-5808 |
| item_10002_relation_14 |
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識別子タイプ |
DOI |
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関連識別子 |
10.1016/j.bbrep.2021.100984 |
| 出版タイプ |
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|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| item_1730428627434 |
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内容記述タイプ |
Other |
|
内容記述 |
査読あり |
1-s2.0-S2405580821000789-main.pdf (2.6 MB)
