| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2025-02-28 |
| タイトル |
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|
タイトル |
Utility of progesterone receptor-ires-Cre to generate conditional knockout mice for uterine study |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 著者 |
Namiki, Takafumi
Kamoshita, Maki
Kageyama, Atsuko
Terakawa, Jumpei
Ito, Junya
Kashiwazaki, Naomi
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| Abstract |
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内容記述タイプ |
Abstract |
|
内容記述 |
In mice, the conditional knockout strategy using the Cre-loxP system is useful for various types of research. The Cre mouse line with progesterone receptor promoter (PgrCre) has been widely used to produce specific uterine gene-deficient mice, but in the Cre line, endogenous Pgr gene is replaced by Cre recombinase gene, which makes the breeding of homozygous mice (PgrCre/Cre) difficult because they are infertile. Yang et al. (2013, https://10.1016/j.cell.2013.04.017) reported the generation of another PgriresCre mouse line that still has endogenous Pgr gene, and they inserted Cre recombinase downstream of the Pgr gene via an internal ribosome entry site (IRES). It is possible that this new PgriresCre line would be useful for uterine research as the mice can be bred as homozygotes (PgriresCre/iresCre). Herein, we confirmed the PgriresCre mice effectively directed recombination in the female reproductive tract and was capable of genetic alteration in the endometrium that enables the studies of its uterine function. Our findings demonstrate that the new PgriresCre mouse line is also useful for the generation of uterine-specific knockout mice. The findings using PgriresCre mouse will contribute to the understanding of reproductive systems and diseases in humans and domestic animals. |
|
言語 |
en |
| 書誌情報 |
Animal Science Journal = Nihon chikusan Gakkaiho
巻 92,
号 1,
p. 1-7,
発行日 2021-08-16
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| 出版者 |
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|
出版者 |
Wiley |
| DOI |
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|
識別子タイプ |
DOI |
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関連識別子 |
10.1111/asj.13615 |
| 著者版フラグ |
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出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| 査読 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
査読あり |
Animal Science Journal - 2021 - Namiki - Utility of progesterone receptor‐ires‐Cre to generate conditional knockout mice.pdf (2.1 MB)
