| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2025-02-28 |
| タイトル |
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タイトル |
Olsalazine inhibits cell proliferation and DNA methylation in canine lymphoid tumor cell lines |
|
言語 |
en |
| 言語 |
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|
言語 |
eng |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 著者 |
Itoh, S
Yamazaki, J
Iwahana, M
Tsukamoto, A
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| Abstract |
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内容記述タイプ |
Abstract |
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内容記述 |
Abnormal DNA methylation is involved in the initiation and progression of lymphoid tumors. Hence, DNA demethylating agents are promising candidate drugs for chemotherapy against these tumors. The salicylic acid derived anti-inflammatory agent, olsalazine, reportedly suppresses DNA methyltransferase in human cells and has the potential to be clinically applied as a DNA demethylating agent. In this study, we investigated the effects of olsalazine on cell proliferation and DNA methylation using canine lymphoid tumor cell lines (CLBL-1, GL-1, and UL-1). Treatment with olsalazine led to significant cell growth inhibition and increased the apoptotic rate in all three cell lines. Treatment with olsalazine reduced the total amount of 5-methylcytosine in genomic DNA, as assessed by enzyme-linked immunosorbent assay. Genome-wide analysis of DNA methylation revealed that 1,801 to 5,626 CpG sites showed decreased DNA methylation levels in three cell lines, including the promoter regions of ADAM23, FES, and CREB3L1 genes. The outcomes of the present study demonstrate that a DNA demethylating agent olsalazine, inhibits cell proliferation and DNA methylation in canine lymphoid tumor cells, suggesting that it can be a candidate drug for the treatment of lymphoid tumors in dogs. |
|
言語 |
en |
| 書誌情報 |
Polish Journal of Veterinary Sciences
巻 24,
号 4,
p. 515-523,
発行日 2021-12-30
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| 出版者 |
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出版者 |
the Polish Academy of Sciences |
| DOI |
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識別子タイプ |
DOI |
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関連識別子 |
10.24425/pjvs.2021.139976 |
| 著者版フラグ |
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出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| 査読 |
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内容記述タイプ |
Other |
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内容記述 |
査読あり |
Olsalazine inhibits cell proliferation and DNA methylation in canine lymphoid tumor cell lines.pdf (836 KB)
