| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2025-02-28 |
| タイトル |
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|
タイトル |
Evaluation of a D-Octaarginine-linked polymer as a transfection tool for transient and stable transgene expression in human and murine cell lines |
|
言語 |
en |
| 言語 |
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|
言語 |
eng |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 著者 |
Sakuma, Saki
Okamoto, Mariko
Matsushita, Nao
Ukawa, Masami
Tomono, Takumi
Kawamoto, Keiko
Ikeda, Teruo
Sakuma, Shinji
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| Abstract |
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内容記述タイプ |
Abstract |
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内容記述 |
Poly(N-vinylacetamide-co-acrylic acid) coupled with d-octaarginine (VP-R8) promotes the cellular uptake of peptides/proteins in vitro; however, details of the transfection efficacy of VP-R8, such as the cell types possessing high gene transfer, are not known. Herein, we compared the ability of VP-R8 to induce the cellular uptake of plasmid DNA in mouse and human cell lines from different tissues and organs. A green fluorescent protein (GFP)-expression plasmid was used as model genetic material, and fluorescence as an indicator of uptake and plasmid-derived protein expression. Three mouse and three human cell lines were incubated with a mixture of plasmid and VP-R8, and fluorescence analysis were performed two days after transfection. To confirm stable transgene expression, we performed drug selection three days after transfection. A commercially available polymer-based DNA transfection reagent (PTR) was used as the transfection control and standard for comparing transgene expression efficiency. In the case of transient transgene expression, slight-to-moderate GFP expression was observed in all cell lines transfected with plasmid via VP-R8; however, transfection efficiency was lower than using the PTR for gene delivery. In the case of stable transgene expression, VP-R8 promoted drug-resistance acquisition more efficiently than the PTR did. Cells that developed drug resistance after VP-R8-mediated gene transfection expressed GFP more efficiently than cells that developed drug resistance after transfection with the PTR. Thus, VP-R8 shows potential as an in vitro or ex vivo nonviral transfection tool for generating cell lines with stable transgene expression. |
|
言語 |
en |
| bibliographic_information |
The Journal of Veterinary Medical Science
巻 84,
号 4,
p. 484-493,
発行日 2022-04-13
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| 出版者 |
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出版者 |
The Japanese Society of Veterinary Science |
| item_10002_source_id_9 |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
0916-7250 |
| item_10002_relation_14 |
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識別子タイプ |
DOI |
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関連識別子 |
10.1292/jvms.21-0647 |
| 出版タイプ |
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|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| item_1730428627434 |
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|
内容記述タイプ |
Other |
|
内容記述 |
査読あり |
84_21-0647.pdf (2.9 MB)
