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History and development of staining methods for skeletal muscle fiber types
https://az.repo.nii.ac.jp/records/2000317
https://az.repo.nii.ac.jp/records/200031765d1cb3c-b42e-410e-a268-2d803bdfc2b8
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
Sawano-37-493-503-2022.pdf (8.4 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||||||
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| 公開日 | 2025-02-28 | |||||||||
| タイトル | ||||||||||
| タイトル | History and development of staining methods for skeletal muscle fiber types | |||||||||
| 言語 | en | |||||||||
| 言語 | ||||||||||
| 言語 | eng | |||||||||
| 資源タイプ | ||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||
| 資源タイプ | journal article | |||||||||
| 著者 |
Sawano, Shoko
× Sawano, Shoko
× Mizunoya, Wataru
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| Abstract | ||||||||||
| 内容記述タイプ | Abstract | |||||||||
| 内容記述 | The contractile and metabolic properties of skeletal muscles depend on the composition of muscle fibers. There are two major fiber types: type 1 and type 2. Type 2 fibers are further subdivided into type 2A, 2X, and 2B fibers. Muscle fiber type composition is an important property that affects sports performance and metabolic ability in humans, and meat quality in domestic animals. In this review, we summarize the history of muscle fiber type classification based on various staining methods for skeletal muscle sections. The history illustrates the development of an experimental method to detect myosin heavy chain (MyHC) proteins, which are the most common marker molecules for muscle fiber type. Metabolic enzymes, such as nicotinamide adenine dinucleotide-tetrazolium reductase and succinate dehydrogenase are also described for histochemical staining combined with myosin ATPase staining. We found an improvement in the quality of antibodies used for immunostaining of MyHC, from polyclonal antibodies to monoclonal antibodies (mAbs) and then to mAbs produced by synthetic peptides as antigens. We believe that the information presented herein will assist researchers in selecting optimal staining methods, dependent on the experimental conditions and purposes. | |||||||||
| 言語 | en | |||||||||
| 書誌情報 |
Histology and histopathology 巻 37, 号 6, p. 493-503, 発行日 2022-06 |
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| 出版者 | ||||||||||
| 出版者 | Sercrisma International SL | |||||||||
| DOI | ||||||||||
| 識別子タイプ | DOI | |||||||||
| 関連識別子 | 10.14670/HH-18-422 | |||||||||
| 著者版フラグ | ||||||||||
| 出版タイプ | VoR | |||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||
| 査読 | ||||||||||
| 内容記述タイプ | Other | |||||||||
| 内容記述 | 査読あり | |||||||||
