| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2025-02-28 |
| タイトル |
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|
タイトル |
Biological features of canine cancer-associated fibroblasts and their influence on cancer cell invasion |
|
言語 |
en |
| 言語 |
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|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 著者 |
Kudo, Ayano
Yoshimoto, Sho
Yoshida, Hiromitsu
Izumi, Yusuke
Takagi, Satoshi
|
| Abstract |
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内容記述タイプ |
Abstract |
|
内容記述 |
Cancer-associated fibroblasts (CAFs) play an essential role in tumor invasion and metastasis. In dogs, the biological features of CAFs have not been well characterized. The purpose of this study was to investigate differences in the biological activities of canine CAFs and normal fibroblasts (NFs), and their influence on the migration and invasion of cancer cells. Canine CAFs and NFs were harvested from surgically-resected malignant epithelial tumor tissues and skin tissues of dogs. A wound-healing assay was conducted to compare the migratory and invasive abilities of canine CAFs and NFs. The results of this study showed that canine CAFs have a greater migratory and invasive ability than NFs. To observe the indirect and direct interactions between fibroblasts and cancer cells, Boyden chamber assay and 3D co-culture with collagen gel were conducted. The number of migrated and infiltrated cancer cells co-cultured with canine CAFs was greater than that with NFs. In the 3D co-culture, cancer cells showed noteworthy proliferation on the surface of gels containing canine CAFs and invasion into the gel. On the other hand, no infiltration of cancer cells into the gel containing NFs was observed. It was suggested that canine CAFs activate migration and invasion of cancer cells and promote the infiltration of cancer cells into collagen gels. |
|
言語 |
en |
| 書誌情報 |
The Journal of veterinary medical science
巻 84,
号 6,
p. 784-791,
発行日 2022-06-10
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| 出版者 |
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|
出版者 |
The Japanese Society of Veterinary Science |
| DOI |
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|
識別子タイプ |
DOI |
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関連識別子 |
10.1292/jvms.22-0041 |
| 著者版フラグ |
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出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| 査読 |
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|
内容記述タイプ |
Other |
|
内容記述 |
査読あり |
84_22-0041.pdf (3.1 MB)
