| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2025-02-03 |
| タイトル |
|
|
タイトル |
Antimicrobial susceptibility of bovine clinical mastitis pathogens in Japan and development of a simplified agar disk diffusion method for clinical practice |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 著者 |
Kawai, Kazuhiro
Kurumisawa, Tomomi
Shinozuka, Yasunori
Higuchi, Hidetoshi
Iwano, Hidetomo
Hayashi, Tomohito
Ozawa, Manao
Koike, Ryoji
Uchiyama, Mariko
|
| Abstract |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
This study aimed to examine the antimicrobial susceptibility of bovine mastitis pathogens in Japan and develop criteria for testing antimicrobial susceptibility using the simplified agar disk diffusion (ADD) method that is currently being used in clinical practice. Milk samples from 1,349 dairy cows with clinical mastitis were collected and cultured. The minimum inhibitory concentrations (MICs) of the antimicrobials were determined for 504 strains of 28 bacteria. Of the gram-positive bacteria, most Staphylococcus spp. were susceptible to penicillin G (PCG), kanamycin (KM), oxytetracycline (OTC), cefazolin (CEZ), pirlimycin, enrofloxacin, and marbofloxacin. Streptococcus spp. and Trueperella pyogenes showed resistance to OTC and KM. Most gram-negative bacteria were resistant to OTC and CEZ and particularly susceptible to fluoroquinolones. To develop the criteria for a disk diffusion test of the simplified ADD method, the relationships between MICs and diameters of inhibition zones (DIZs) were analyzed and compared with the conventional method. The susceptibility breakpoints of several antimicrobials were lower for both gram-positive and gram-negative bacteria. Particularly for gram-positive bacteria, the application of the new criteria lowers the breakpoint for PCG, suggesting that the use of PCG instead of CEZ may increase. The results suggest that use of these criteria for the simplified ADD method may lead to appropriate antimicrobial choice and consequently the appropriate use of antimicrobials in clinical practice. |
|
言語 |
en |
| 書誌情報 |
The Journal of Veterinary Medical Science
巻 85,
号 2,
p. 143-148,
発行日 2023
|
| 出版者 |
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|
出版者 |
The Japanese Society of Veterinary Science |
| ISSN |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
0916-7250 |
| DOI |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
10.1292/jvms.21-0450 |
| 著者版フラグ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| 査読 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
査読あり |
jvms-85-143.pdf (578 KB)
