| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2025-02-03 |
| タイトル |
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|
タイトル |
Successful production of offspring derived from mouse zygotes vitrified with carboxylated ε-poly-L-lysine and polyvinyl alcohol without serum |
|
言語 |
en |
| 言語 |
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|
言語 |
eng |
| 資源タイプ |
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|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 著者 |
Ishii, Midori
Kamoshita, Maki
Kurihara, Yoshihiro
Matsumura, Kazuaki
Hyon, Suong-Hyu
Ito, Junya
Kashiwazaki, Naomi
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| Abstract |
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内容記述タイプ |
Abstract |
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内容記述 |
The vitrification of zygotes is important for their use as donors for generating genome-edited mice. We previously reported the successful vitrification of mouse zygotes using carboxylated ε-poly-L-lysine (COOH-PLL). However, this vitrification solution contains fetal calf serum (FCS), which contains unknown factors and presents risks of pathogenic viral and microbial contamination. In this study, we examined whether polyvinyl alcohol (PVA) can be used as an alternative to FCS in vitrification solutions for mouse zygotes. When COOH-PLL was added to the vitrification solutions, zygotes vitrified with solutions containing 0.01% PVA (PV0.01) and those vitrified in a control solution containing FCS (75.6%) developed into blastocysts (78.4%). In addition, there were no significant differences in the ability to develop to term between the control solution (46.6%) and PV0.01 (44.1%) groups. In conclusion, we clearly demonstrated that PVA can replace FCS in our vitrification solution supplemented with COOH-PLL for mouse zygotes. |
|
言語 |
en |
| 書誌情報 |
The Journal of reproduction and development
巻 69,
号 1,
p. 53-55,
発行日 2023-02-08
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| 出版者 |
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出版者 |
The Society for Reproduction and Development |
| DOI |
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|
識別子タイプ |
DOI |
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関連識別子 |
10.1262/jrd.2022-121 |
| 著者版フラグ |
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出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| 査読 |
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|
内容記述タイプ |
Other |
|
内容記述 |
査読あり |
jrd-69-053.pdf (646 KB)
