| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2025-02-03 |
| タイトル |
|
|
タイトル |
Xenopus laevis Oocyte Array Fluidic Device Integrated with Microelectrodes for A Compact Two-Electrode Voltage Clamping System |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 著者 |
Misawa, Nobuo
Tomida, Mitsuyoshi
Murakami, Yuji
Mitsuno, Hidefumi
Kanzaki, Ryohei
|
| Abstract |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
We report on a compact two-electrode voltage clamping system composed of microfabricated electrodes and a fluidic device for Xenopus laevis oocytes. The device was fabricated by assembling Si-based electrode chips and acrylic frames to form fluidic channels. After the installation of Xenopus oocytes into the fluidic channels, the device can be separated in order to measure changes in oocyte plasma membrane potential in each channel using an external amplifier. Using fluid simulations and experiments, we investigated the success rates of Xenopus oocyte arrays and electrode insertion with respect to the flow rate. We successfully located each oocyte in the array and detected oocyte responses to chemical stimuli using our device. |
|
言語 |
en |
| 書誌情報 |
Sensors
巻 23,
号 5,
p. 2370-2370,
発行日 2023-02-21
|
| 出版者 |
|
|
出版者 |
MDPI AG |
| DOI |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
10.3390/s23052370 |
| 著者版フラグ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| 査読 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
査読あり |
sensors-23-02370-v5.pdf (2.5 MB)
