| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2025-01-24 |
| タイトル |
|
|
タイトル |
Generation of a recombinant temperature-sensitive influenza D virus |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 著者 |
Ishida, Hiroho
Murakami, Shin
Kamiki, Haruhiko
Matsugo, Hiromichi
Katayama, Misa
Sekine, Wataru
Ohira, Kosuke
Takenaka-Uema, Akiko
Horimoto, Taisuke
|
| Abstract |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Influenza D virus (IDV) is a causative agent of the bovine respiratory disease complex (BRDC), which is the most common and costly disease affecting the cattle industry. For developing a candidate vaccine virus against IDV, we sought to produce a temperature-sensitive strain, similar to the live attenuated, cold-adapted vaccine strain available against the influenza A virus (IAV). To this end, we produced a recombinant IDV (designated rD/OK-AL) strain by introducing mutations responsible for the adaptation of the IAV vaccine strain to cold conditions and conferring sensitivity to high temperatures into PB2 and PB1 proteins using reverse genetics. The rD/OK-AL strain grew efficiently at 33 °C but did not grow at 37 °C in the cell culture, indicating its high-temperature sensitivity. In mice, rD/OK-AL was attenuated following intranasal inoculation. It mediated the production of high levels of antibodies against IDV in the serum. When the rD/OK-AL-inoculated mice were challenged with the wild-type virus, the virus was not detected in respiratory organs after the challenge, indicating complete protection against IDV. These results imply that the rD/OK-AL might be a potential candidate for the development of live attenuated vaccines for IDV that can be used to control BRDC. |
|
言語 |
en |
| 書誌情報 |
Scientific reports
巻 13,
号 1,
p. 3806-3806,
発行日 2023-03-07
|
| 出版者 |
|
|
出版者 |
Springer Nature |
| DOI |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
10.1038/s41598-023-30942-z |
| 著者版フラグ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| 査読 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
査読あり |
s41598-023-30942-z.pdf (3.2 MB)
