| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2025-01-24 |
| タイトル |
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|
タイトル |
Up- and Downregulated Genes after Long-Term Muscle Atrophy Induced by Denervation in Mice Detected Using RNA-Seq |
|
言語 |
en |
| 言語 |
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|
言語 |
eng |
| 資源タイプ |
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|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 著者 |
Sawano, Shoko
Fukushima, Misaki
Akasaka, Taiki
Nakamura, Mako
Tatsumi, Ryuichi
Ikeuchi, Yoshihide
Mizunoya, Wataru
|
| Abstract |
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内容記述タイプ |
Abstract |
|
内容記述 |
Skeletal muscle atrophy occurs rapidly as a result of inactivity. Although there are many reports on changes in gene expression during the early phase of muscle atrophy, the patterns of up-and downregulated gene expression after long-term and equilibrated muscle atrophy are poorly understood. In this study, we comprehensively examined the changes in gene expression in long-term denervated mouse muscles using RNA-Seq. The murine right sciatic nerve was denervated, and the mice were housed for five weeks. The cross-sectional areas of the hind limb muscles were measured using an X-ray CT system 35 days after denervation. After 28 d of denervation, the cross-sectional area of the muscle decreased to approximately 65% of that of the intact left muscle and reached a plateau. Gene expression in the soleus and extensor digitorum longus (EDL) muscles on the 36th day was analyzed using RNA-Seq and validated using RT-qPCR. RNA-Seq analysis revealed that three genes—Adora1, E230016M11Rik, and Gm10718—were upregulated and one gene—Gm20515—was downregulated in the soleus muscle; additionally, four genes—Adora1, E230016M11Rik, Pigh, and Gm15557—were upregulated and one gene—Fzd7—was downregulated in the EDL muscle (FDR < 0.05). Among these genes, E230016M11Rik, one of the long non-coding RNAs, was significantly upregulated in both the muscles. These findings indicate that E230016M11Rik could be a candidate gene for the maintenance of atrophied skeletal muscle size and an atrophic state. |
|
言語 |
en |
| 書誌情報 |
Life (Basel, Switzerland)
巻 13,
号 5,
p. 1-13,
発行日 2023-04-29
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| 出版者 |
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出版者 |
MDPI |
| DOI |
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|
識別子タイプ |
DOI |
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関連識別子 |
10.3390/life13051111 |
| 著者版フラグ |
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|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| 査読 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
査読あり |
life-13-01111.pdf (2.2 MB)
