| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2025-01-24 |
| タイトル |
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|
タイトル |
A combined treatment with progesterone, anti-inhibin serum, and equine chorionic gonadotropin improves number of ovulated oocytes in young C57BL/6J mice |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 著者 |
Kageyama, Atsuko
Tsuchiya, Mizuho
Terakawa, Jumpei
Ito, Junya
Kashiwazaki, Naomi
|
| Abstract |
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内容記述タイプ |
Abstract |
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内容記述 |
Superovulation procedures are routinely and widely used in mouse reproductive technology. Previous studies have shown that a large number of oocytes can be obtained from adult mice (> 10 weeks old) using a combined treatment with progesterone (P4) and anti-inhibin serum (AIS). However, these effects have not been fully investigated in young (4 weeks) C57BL/6J mice. Here, we found that a modified superovulation protocol (combined treatment with P4, AIS, eCG (equine chorionic gonadotropin), and hCG (human chorionic gonadotropin); P4D2-Ae-h) improved the number of oocytes compared to the control (eCG and hCG) (39.7 vs. 21.3 oocytes/mouse). After in vitro fertilization, pronuclear formation rates were 69.3% (P4D2-Ae-h group) and 66.2% (control group). After embryo transfer, 46.4% (116/250) of the embryos in the P4D2-Ae-h group successfully developed to term, which was comparable to the control group (42.9%; 123/287 embryos). In conclusion, our protocol (P4D2-Ae-h) was effective for superovulation in young C57BL/6J mice. |
|
言語 |
en |
| 書誌情報 |
The Journal of reproduction and development
巻 69,
号 4,
p. 223-226,
発行日 2023-06-17
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| 出版者 |
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|
出版者 |
The Society for Reproduction and Development |
| DOI |
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|
識別子タイプ |
DOI |
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関連識別子 |
10.1262/jrd.2023-036 |
| 著者版フラグ |
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出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| 査読 |
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|
内容記述タイプ |
Other |
|
内容記述 |
査読あり |
Journal of Reproduction and Development.pdf (800 KB)
