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Characterization of a nuclear transport factor 2-like domain-containing protein in Plasmodium berghei
https://az.repo.nii.ac.jp/records/2000192
https://az.repo.nii.ac.jp/records/200019277017b01-f36a-478e-8b5b-ea2c33ee25aa
| 名前 / ファイル | ライセンス | アクション |
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s12936-024-04839-9.pdf (1.5 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||||||||||
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| 公開日 | 2024-12-13 | |||||||||||||
| タイトル | ||||||||||||||
| タイトル | Characterization of a nuclear transport factor 2-like domain-containing protein in Plasmodium berghei | |||||||||||||
| 言語 | en | |||||||||||||
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| 言語 | eng | |||||||||||||
| 資源タイプ | ||||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||
| 資源タイプ | journal article | |||||||||||||
| 著者 |
Niikura, Mamoru
× Niikura, Mamoru
× Fukutomi, Toshiyuki
× Mitobe, Jiro
× Kobayashi, Fumie
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| Abstract | ||||||||||||||
| 内容記述タイプ | Abstract | |||||||||||||
| 内容記述 | Background Plasmodium lacks an mRNA export receptor ortholog, such as yeast Mex67. Yeast Mex67 contains a nuclear transport factor 2 (NTF2)-like domain, suggesting that NTF2-like domain-containing proteins might be associated with mRNA export in Plasmodium. In this study, the relationship between mRNA export and an NTF2-like domain-containing protein, PBANKA_1019700, was investigated using the ANKA strain of rodent malaria parasite Plasmodium berghei. Methods The deletion mutant Δ1019700 was generated by introducing gene-targeting vectors into the P. berghei ANKA genome, and parasite growth and virulence were examined. To investigate whether PBANKA_1019700 is involved in mRNA export, live-cell fluorescence imaging and immunoprecipitation coupled to mass spectrometry (IP-MS) were performed using transgenic parasites expressing fusion proteins (1019700::mCherry). Results Deletion of PBANKA_1019700 affected the sexual phase but not the asexual phase of malaria parasites. Live-cell fluorescence imaging showed that PBANKA_1019700 localizes to the cytoplasm. Moreover, IP-MS analysis of 1019700::mCherry indicated that PBANKA_1019700 interacts with ubiquitin-related proteins but not nuclear proteins. Conclusions PBANKA_1019700 is a noncanonical NTF2-like superfamily protein. |
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| 言語 | en | |||||||||||||
| 書誌情報 |
Malaria journal 巻 23, 号 1, p. 13-13, 発行日 2024-01-09 |
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| 出版者 | ||||||||||||||
| 出版者 | Springer Nature | |||||||||||||
| DOI | ||||||||||||||
| 識別子タイプ | DOI | |||||||||||||
| 関連識別子 | 10.1186/s12936-024-04839-9 | |||||||||||||
| 著者版フラグ | ||||||||||||||
| 出版タイプ | VoR | |||||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||||
| 査読 | ||||||||||||||
| 内容記述タイプ | Other | |||||||||||||
| 内容記述 | 査読あり | |||||||||||||
