| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2024-12-10 |
| タイトル |
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タイトル |
The etherase system of Novosphingobium sp. MBES04 functions as a sensor of lignin fragments through phenylpropanone production to induce specific transcriptional responses |
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言語 |
en |
| 言語 |
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|
言語 |
eng |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 著者 |
Kumagawa, Eri
Katsumata, Madoka
Nishimura, Hiroshi
Watanabe, Takashi
Ishii, Shun'ichi
Ohta, Yukari
|
| Abstract |
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内容記述タイプ |
Abstract |
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内容記述 |
The MBES04 strain of Novosphingobium accumulates phenylpropanone monomers as end-products of the etherase system, which specifically and reductively cleaves the β-O-4 ether bond (a major bond in lignin molecules). However, it does not utilise phenylpropanone monomers as an energy source. Here, we studied the response to the lignin-related perturbation to clarify the physiological significance of its etherase system. Transcriptome analysis revealed two gene clusters, each consisting of four tandemly linked genes, specifically induced by a lignin preparation extracted from hardwood (Eucalyptus globulus) and a β-O-4-type lignin model biaryl compound, but not by vanillin. The most strongly induced gene was a 2,4'-dihydroxyacetophenone dioxygenase-like protein, which leads to energy production through oxidative degradation. The other cluster was related to multidrug resistance. The former cluster was transcriptionally regulated by a common promoter, where a phenylpropanone monomer acted as one of the effectors responsible for gene induction. These results indicate that the physiological significance of the etherase system of the strain lies in its function as a sensor for lignin fragments. This may be a survival strategy to detect nutrients and gain tolerance to recalcitrant toxic compounds, while the strain preferentially utilises easily degradable aromatic compounds with lower energy demands for catabolism. |
|
言語 |
en |
| 書誌情報 |
Environmental Microbiology Reports
巻 16,
号 1,
発行日 2024-02
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| 出版者 |
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出版者 |
WILEY |
| DOI |
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識別子タイプ |
DOI |
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関連識別子 |
10.1111/1758-2229.13210 |
| 著者版フラグ |
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出版タイプ |
VoR |
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出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| Other |
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内容記述タイプ |
Other |
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内容記述 |
査読あり |
Environ Microbiol Rep - 2023 - Kumagawa - The etherase system of Novosphingobium sp MBES04 functions as a sensor of lignin.pdf (3.9 MB)
