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Molecular form identification of anterior pituitary gland-secreted prolactin in chicken
https://az.repo.nii.ac.jp/records/2000182
https://az.repo.nii.ac.jp/records/20001826046ae33-7ba5-40fa-b62a-1072eabdee13
| Item type | 学術雑誌論文 / Journal Article(1) | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| 公開日 | 2024-12-09 | |||||||||
| タイトル | ||||||||||
| タイトル | Molecular form identification of anterior pituitary gland-secreted prolactin in chicken | |||||||||
| 言語 | en | |||||||||
| 言語 | ||||||||||
| 言語 | eng | |||||||||
| 資源タイプ | ||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||
| 資源タイプ | journal article | |||||||||
| 著者 |
Kansaku, Norio
× Kansaku, Norio
× Ohkubo, Takeshi
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| Abstract | ||||||||||
| 内容記述タイプ | Abstract | |||||||||
| 内容記述 | Endocrine changes during bird reproduction are well documented. Prolactin (PRL) exhibits a strong relationship between incubation and broody behavior. The molecular forms of PRL in the anterior pituitary gland during the reproductive cycle have already been previously identified but not those in the secreted form. To identify the molecular forms of secreted PRL during the reproductive cycle, we thus monitored the physiological status and incubation behavior of 10 Silkie hens by a video recording system over 1–2 years. Nine out of ten mature hens exhibited incubation behavior multiple times during the experiment. Ten hens demonstrated two interesting features. In a typical clutch, hens spent 10–15 min in the nest to lay an egg. Once they spent over 1 h in the nest, the nest occupancy increased incrementally. This shift in the nest occupancy occurred 7–10 days before the incubation onset and was highly repeatable. Based on the behavior of the hens, we cultured the anterior pituitary gland during four stages (premature non-laying, laying, trans, and incubation) with physiological PRL-releasing factor, vasoactive intestinal peptide (VIP). Based on our two-dimensional protein analysis, glycosylated PRL (G-PRL) displayed several isoforms with varying isoelectric points (pI), whereas we could detect one primary signal for non-glycosylated PRL (NG-PRL). However, 3–4 NG-PRL isoforms were detected in the anterior pituitary gland. These results suggested that secreted PRL, especially from the trans and incubation stages, contains various isoforms and it is post-translationally glycosylated and phosphorylated. | |||||||||
| 言語 | en | |||||||||
| 書誌情報 |
General and Comparative Endocrinology 巻 346, p. 114415-114415, 発行日 2024-01 |
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| 出版者 | ||||||||||
| 出版者 | Elsevier BV | |||||||||
| ISSN | ||||||||||
| 収録物識別子タイプ | ISSN | |||||||||
| 収録物識別子 | 0016-6480 | |||||||||
| DOI | ||||||||||
| 識別子タイプ | DOI | |||||||||
| 関連識別子 | 10.1016/j.ygcen.2023.114415 | |||||||||
| 査読 | ||||||||||
| 内容記述タイプ | Other | |||||||||
| 内容記述 | 査読あり | |||||||||
