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環境汚染物質代謝に関与する新規ラッカーゼのスクリーニング
https://az.repo.nii.ac.jp/records/5066
https://az.repo.nii.ac.jp/records/506641373272-83dd-49f7-a58f-fe938afc0e7d
名前 / ファイル | ライセンス | アクション |
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bull_jau_vol15-16-277.pdf (565.6 kB)
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Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2007-01-01 | |||||
タイトル | ||||||
タイトル | 環境汚染物質代謝に関与する新規ラッカーゼのスクリーニング | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Screening of novel laccases degrading the environmental pollutants | |||||
言語 | ||||||
言語 | jpn | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | departmental bulletin paper | |||||
ページ属性 | ||||||
内容記述タイプ | Other | |||||
内容記述 | P(論文) | |||||
記事種別 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 特集 | |||||
Type | ||||||
内容記述タイプ | Other | |||||
内容記述 | FEATURE ARTICLES | |||||
著者 |
其木, 茂則
× 其木, 茂則× Sonoki, Shigenori |
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著者ID | ||||||
000120 | ||||||
Author ID | ||||||
000120 | ||||||
所属機関 | ||||||
麻布大学大学院環境保健学研究科環境保健科学専攻環境化学 | ||||||
Institution or Company | ||||||
Environmental Chemistry, Course of Environmental Health Science, Graduate School of Environmental Health, Azabu university | ||||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | 白色腐朽葉の菌種間,また同一の菌種内で互いに酵素化学的な性質の異なるアイソフォームが存在し,水酸化PCBの代謝性も異なることがわかった。T. versicolor産生ラッカーゼにおける水酸化PCB分解では,陰イオン交換樹脂未吸着両分との反応では,高塩赤化水酸化PCBは酸性側よりも中性側で分解率が高く,陰イオン交換樹脂吸着両分との反応では,低塩素化水酸化PCBについても同様の動向を示すことがわかった。さらに,同じ四塩素化水酸化PCBでも塩素の結合位置の違いにより,分解率に差が生じることが明らかとなった。 | |||||
Abstract | ||||||
内容記述タイプ | Other | |||||
内容記述 | Several laccase isoforms showing the different enzymatic property were obtained from the culture fluid of some species of white-rot fungus, Trametes versicolor, T. cervina, T. conchifer and T. elegans. Each strain produced two kinds of laccases in the culture, one of which passed through the ion-exchange DEAE-Sepharose column at pH 5.0 (fraction A) and the other adsorbed the column (fraction B). Using 2, 2'-azinobis (3-ethylbenzothiazoline-6-sulfonate) (ABTS) as the substrate for laccase, the enzymatic property such as the optimum pH, the optimum temperature or the thermostability was investigated. As a result, every laccase from each strain had the higher activity in the acidic range, pH 3.8-4.4, and at the higher temperature, 60℃~80℃. Every laccase except the fraction B from T. conchifer and T. elegans showed the higher stability until 60℃; however, the fraction B still reserved the higher activity at 80℃. The degradation of hydroxyPCBs which are suspected of endocrine disruptors in the in vitro incubation with laccases was examined. Overall, the fraction A has a higher activity to reduce each level of hydroxyPCBs than the fraction B. In equally chlorinated 4-hydroxy-tetrachlorobiphenyl congeners, 4-hydroxy-2',3,5,5'-tetrachlorobiphenyl was more degraded than 4-hydroxy-2',3',4',5'-tetrachlorobiphenyl. It seems that the chlorine binding position is responsible for the rising susceptibility to the enzymatic degradation of hydroxyPCBs. | |||||
書誌情報 |
麻布大学雑誌 en : Journal of Azabu University 巻 15/16, p. 277-281, 発行日 2008-03-31 |
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出版者 | ||||||
出版者 | 麻布大学 | |||||
Publisher | ||||||
出版者 | Azabu University | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1346-5880 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA11561554 | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
text version | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
format | ||||||
内容記述タイプ | Other | |||||
内容記述 | application/pdf |