WEKO3
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乳酸菌における多剤耐性遺伝子(lmrA・lmrP)の検索とクローニング
https://az.repo.nii.ac.jp/records/4377
https://az.repo.nii.ac.jp/records/43775eec71e6-f5b5-4fd1-b483-72a67e80a4e0
名前 / ファイル | ライセンス | アクション |
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bull_jau_vol3-4-059 (1.1 MB)
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Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2001-01-01 | |||||
タイトル | ||||||
タイトル | 乳酸菌における多剤耐性遺伝子(lmrA・lmrP)の検索とクローニング | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Detection and Cloning of Multidmg Resistance Genes (lmrA・lmrP) in Several Lactic Acid Bacteria | |||||
言語 | ||||||
言語 | jpn | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | multidrug resistance genes | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | LmrA and LmrP | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | lactic acid bacteria | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | expression vector | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | departmental bulletin paper | |||||
ページ属性 | ||||||
内容記述タイプ | Other | |||||
内容記述 | P(論文) | |||||
記事種別 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 原著論文 | |||||
Type | ||||||
内容記述タイプ | Other | |||||
内容記述 | ORIGINAL ARTICLE | |||||
著者 |
鈴木, 武人
× 鈴木, 武人× 森田, 英利× 松下, 瑠美× 橋爪, 克仁× 坂田, 亮一× 舘, 鄰× SUZUKI, Takehito× MORITA, Hidetoshi× MATSUSHITA, Rumi× HASHIZUME, Katsuhito× SAKATA, Ryoichi× TACHI, Chikashi |
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著者ID | ||||||
000395 | ||||||
著者ID | ||||||
000247 | ||||||
著者ID | ||||||
- | ||||||
著者ID | ||||||
- | ||||||
著者ID | ||||||
000054 | ||||||
著者ID | ||||||
- | ||||||
Author ID | ||||||
000395 | ||||||
Author ID | ||||||
000247 | ||||||
Author ID | ||||||
- | ||||||
Author ID | ||||||
- | ||||||
Author ID | ||||||
000054 | ||||||
Author ID | ||||||
- | ||||||
所属機関 | ||||||
麻布大学獣医学部 | ||||||
所属機関 | ||||||
麻布大学獣医学部 | ||||||
所属機関 | ||||||
麻布大学獣医学部 | ||||||
所属機関 | ||||||
宝酒造(株)遺伝子解析センター | ||||||
所属機関 | ||||||
麻布大学獣医学部 | ||||||
所属機関 | ||||||
麻布大学獣医学部 | ||||||
Institution or Company | ||||||
School of Veterinary Medicine, Azabu University | ||||||
Institution or Company | ||||||
School of Veterinary Medicine, Azabu University | ||||||
Institution or Company | ||||||
School of Veterinary Medicine, Azabu University | ||||||
Institution or Company | ||||||
Gene Analysis Center, Takara Syuzo Inc. | ||||||
Institution or Company | ||||||
School of Veterinary Medicine, Azabu University | ||||||
Institution or Company | ||||||
School of Veterinary Medicine, Azabu University | ||||||
Abstract | ||||||
内容記述タイプ | Other | |||||
内容記述 | The genes, lmrA and lmrP, are identified as multidrug resistant genes in Lactococcus lactis subsp lactis MG1363, and these genes express LmrA and LmrP which are multidrug transporters (Konings et al., 1994-2000^<1-14)>). Multidrug transporters are a representative mechanism of multidrug resistance in bacteria and mammals. The amino acid sequence of LmrA showed 30% homology to the N- and C-terminal halves of the human multidrug resistant P-glycoprotein MDR1. And especially the sequence identities of ATP binding cassette domain of these protein are high (48% and 43% identical, respectively). In this study, attempts were made to confirm the presence of multidrug resistant genes in Lactococcus and Lactobacillus, which are lactic acid bacteria. The complete genomic DNA sequences of both lmrA and lmrP were isolated from 10 strains of Lactococcus lactis subsp. lactis by PCR amplification, whereas the presence of the homologous genomic DNA sequences genes was not confirmed in Lactococcus lactis subsp. cremoris and Lactobacillus. Vector pCAGGS (Niwa et al., 1991^<15)>) was used as the expression vector for lmrA in mammalian cells in this study. The PCR products of lmrA was cloned into pCAGGS in XhoI site, and pCAGGS-lmrA (7,873 bp) was constructed. | |||||
書誌情報 |
麻布大学雑誌 en : Journal of Azabu University 巻 3/4, p. 59-69, 発行日 2002-03-31 |
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出版者 | ||||||
出版者 | 麻布大学 | |||||
Publisher | ||||||
出版者 | Azabu University | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1346-5880 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA11561468 | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
text version | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
format | ||||||
内容記述タイプ | Other | |||||
内容記述 | application/pdf |